Description: Rabbit polyclonal antibody to FOXD3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXD3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: FOXD3 Alternative Names: HFH2; Forkhead box protein D3; HNF3/FH transcription factor genesisEntrez Gene (Human): 27022Entrez Gene (Mouse) : 15221SwissProt (Human): Q9UJU5SwissProt (Mouse) : Q61060SwissProt (Rat) : Q63245Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FOXD3 expression in K562 (A), Jurkat (B), C6 (C), Raw264.7 (D) whole cell lysates.
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Immunohistochemical analysis of FOXD3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development