Description: Rabbit polyclonal antibody to c-FOS (Phospho-S362) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S362 of human c-FOS protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), FC (1/100 - 1/200) Gene Symbol: FOS Alternative Names: G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7Entrez Gene (Human): 2353Entrez Gene (Mouse) : 14281Entrez Gene (Rat) : 314322SwissProt (Human): P01100SwissProt (Mouse) : P01101SwissProt (Rat) : P12841Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of c-FOS (Phospho-S362) expression in HepG2 (A), HeLa Adriamycin-treated (B) whole cell lysates.
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Immunohistochemical analysis of c-FOS (Phospho-S362) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
A Role for the Immediate Early Gene Product c-fos in Imprinting T Cells with Short-Term Memory for Signal Summation