Description: Rabbit polyclonal antibody to CD142 (Phospho-S290) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S290 of human CD142 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: F3 Alternative Names: Tissue factor; TF; Coagulation factor III; Thromboplastin; CD142Entrez Gene (Human): 2152SwissProt (Human): P13726Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD142 (Phospho-S290) expression in mouse brain (A), mouse lung (B), mouse liver (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of CD142 (Phospho-S290) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.