Description: Rabbit polyclonal antibody to ETV4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ETV4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ETV4 Alternative Names: E1AF; PEA3; ETS translocation variant 4; Adenovirus E1A enhancer-binding protein; E1A-F; Polyomavirus enhancer activator 3 homolog; Protein PEA3Entrez Gene (Human): 2118Entrez Gene (Mouse) : 18612SwissProt (Human): P43268SwissProt (Mouse) : P28322Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ETV4 expression in HeLa (A), Raw264.7 (B), PC12 (C) whole cell lysates.
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  Immunohistochemical analysis of ETV4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.