Description: Rabbit polyclonal antibody to PE2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PE2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ERF Alternative Names: ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2Entrez Gene (Human): 2077Entrez Gene (Mouse) : 13875SwissProt (Human): P50548SwissProt (Mouse) : P70459Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PE2 expression in rat heart (A), rat testis (B) whole cell lysates.
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Immunohistochemical analysis of PE2 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.