Description: Rabbit polyclonal antibody to HER2 (Phospho-Y1221/Y1222) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1221/Y1222 of human HER2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100) Gene Symbol: ERBB2 Alternative Names: HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340Entrez Gene (Human): 2064SwissProt (Human): P04626Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HER2 (Phospho-Y1221/Y1222) expression in HepG2 EGF-treated (A), A431 EGF-treated (B) whole cell lysates.
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Immunohistochemical analysis of HER2 (Phospho-Y1221/Y1222) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.