Description: Rabbit polyclonal antibody to MEN Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MEN. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ELL Alternative Names: C19orf17; RNA polymerase II elongation factor ELL; Eleven-nineteen lysine-rich leukemia proteinEntrez Gene (Human): 8178Entrez Gene (Mouse) : 13716SwissProt (Human): P55199SwissProt (Mouse) : O08856Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MEN expression in mouse lung (A), mouse testis (B), rat lung (C), rat testis (D) whole cell lysates.
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Immunohistochemical analysis of MEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb)