Anti-Ephrin A5 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to Ephrin A5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ephrin A5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200) Gene Symbol: EFNA5 Alternative Names: EPLG7; LERK7; Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7Entrez Gene (Human): 1946Entrez Gene (Mouse) : 13640Entrez Gene (Rat) : 116683SwissProt (Human): P52803SwissProt (Mouse) : O08543SwissProt (Rat) : P97605Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ephrin A5 expression in mouse heart (A), rat heart (B), rat kidney (C) whole cell lysates. -
Immunohistochemical analysis of Ephrin A5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of Ephrin A5 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice
Polysialylated NCAM and EphrinA/EphA Regulate Synaptic Development of GABAergic Interneurons in Prefrontal Cortex
Eph/ephrin interactions modulate muscle satellite cell motility and patterning
Pathway-specific engagement of ephrinA5-EphA4/EphA5 system of the substantia nigra pars reticulata in cocaine-induced responses
Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling