Description: Rabbit polyclonal antibody to DUSP22 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DUSP22. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: DUSP22 Alternative Names: JSP1; LMWDSP2; MKPX; Dual specificity protein phosphatase 22; JNK-stimulatory phosphatase-1; JSP-1; Low molecular weight dual specificity phosphatase 2; LMW-DSP2; Mitogen-activated protein kinase phosphatase x; MAP kinase phosphatase x; MKP-xEntrez Gene (Human): 56940Entrez Gene (Mouse) : 105352SwissProt (Human): Q9NRW4SwissProt (Mouse) : Q99N11Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DUSP22 expression in SHSY5Y (A), mouse liver (B), mouse heart (C), rat liver (D), rat heart (E) whole cell lysates.
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Immunofluorescent analysis of DUSP22 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).