Description: Rabbit polyclonal antibody to CRMP2 (Phospho-S522) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S522 of human CRMP2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DPYSL2 Alternative Names: CRMP2; ULIP2; Dihydropyrimidinase-related protein 2; DRP-2; Collapsin response mediator protein 2; CRMP-2; N2A3; Unc-33-like phosphoprotein 2; ULIP-2Entrez Gene (Human): 1808Entrez Gene (Mouse) : 12934Entrez Gene (Rat) : 25416SwissProt (Human): Q16555SwissProt (Mouse) : O08553SwissProt (Rat) : P47942Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CRMP2 (Phospho-S522) expression in A375 (A), MCF7 (B), PC12 (C), AML12 (D) whole cell lysates.
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Immunohistochemical analysis of CRMP2 (Phospho-S522) staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.