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Anti-DGK epsilon Antibody CPA3898
  • Western blot analysis of DGK epsilon expression in HEK293T (A), rat spleen (B) whole cell lysates.
  • Immunohistochemical analysis of DGK epsilon staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-DGK epsilon Antibody
Cat No: CPA3898
Source: Rabbit
Reactivity: H, R, Mk
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to DGK epsilon
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DGK epsilon. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol: DGKE
Alternative Names: DAGK5; Diacylglycerol kinase epsilon; DAG kinase epsilon; Diglyceride kinase epsilon; DGK-epsilon
Entrez Gene (Human): 8526
Entrez Gene (Mouse) : 56077
SwissProt (Human): P52429
SwissProt (Mouse) : Q9R1C6
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of DGK epsilon expression in HEK293T (A), rat spleen (B) whole cell lysates.
  • Immunohistochemical analysis of DGK epsilon staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome
Journal Nat Genet
IF 30.8
Application
Reactivity
PMID 23542698
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