Description: Rabbit polyclonal antibody to DGK epsilon Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DGK epsilon. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DGKE Alternative Names: DAGK5; Diacylglycerol kinase epsilon; DAG kinase epsilon; Diglyceride kinase epsilon; DGK-epsilonEntrez Gene (Human): 8526Entrez Gene (Mouse) : 56077SwissProt (Human): P52429SwissProt (Mouse) : Q9R1C6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DGK epsilon expression in HEK293T (A), rat spleen (B) whole cell lysates.
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Immunohistochemical analysis of DGK epsilon staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome