Anti-Cytochrome P450 2S1 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to Cytochrome P450 2S1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2S1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: CYP2S1 Alternative Names: Cytochrome P450 2S1; CYPIIS1Entrez Gene (Human): 29785Entrez Gene (Mouse) : 74134SwissProt (Human): Q96SQ9SwissProt (Mouse) : Q9DBX6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cytochrome P450 2S1 expression in U87MG (A), A549 (B), AML12 (C), mouse colon (D) whole cell lysates. -
Immunohistochemical analysis of Cytochrome P450 2S1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of Cytochrome P450 2S1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CYP2S1 and CYP2W1 mediate 2-(3,4-dimethoxyphenyl)-5-fluorobenzothiazole (GW-610, NSC 721648) sensitivity in breast and colorectal cancer cells
Restitution of tumor suppressor microRNAs using a systemic nanovector inhibits pancreatic cancer growth in mice