Description: Rabbit polyclonal antibody to ICER Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ICER. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CREM Alternative Names: cAMP-responsive element modulator; Inducible cAMP early repressor; ICEREntrez Gene (Human): 1390Entrez Gene (Mouse) : 12916Entrez Gene (Rat) : 25620SwissProt (Human): Q03060SwissProt (Mouse) : P27699SwissProt (Rat) : Q03061Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ICER expression in mouse kidney (A), rat lung (B) whole cell lysates.
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Immunohistochemical analysis of ICER staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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