Description: Rabbit polyclonal antibody to CLOCK Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CLOCK. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CLOCK Alternative Names: BHLHE8; KIAA0334; Circadian locomoter output cycles protein kaput; hCLOCK; Class E basic helix-loop-helix protein 8; bHLHe8Entrez Gene (Human): 9575Entrez Gene (Mouse) : 12753Entrez Gene (Rat) : 60447SwissProt (Human): O15516SwissProt (Mouse) : O08785SwissProt (Rat) : Q9WVS9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CLOCK expression in HEK293T (A), H446 (B) whole cell lysates.
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  Immunohistochemical analysis of CLOCK staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.