Anti-CHK2 (Phospho-T383) Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to CHK2 (Phospho-T383) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T383 of human CHK2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: CHEK2 Alternative Names: CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2Entrez Gene (Human): 11200Entrez Gene (Mouse) : 50883SwissProt (Human): O96017SwissProt (Mouse) : Q9Z265Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CHK2 (Phospho-T383) expression in HEK293T (A), HEK293T-UV-30min (B) whole cell lysates. -
Immunofluorescent analysis of CHK2 (Phospho-T383) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Chk2 prevents mitotic exit when the majority of kinetochores are unattached
Exposure to bisphenol A disrupts meiotic progression during spermatogenesis in adult rats through estrogen-like activity
Geminin is required for zygotic gene expression at the Xenopus mid-blastula transition
Interdependent phosphorylation within the kinase domain T-loop Regulates CHK2 activity