Description: Rabbit polyclonal antibody to CFTR Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CFTR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CFTR Alternative Names: ABCC7; Cystic fibrosis transmembrane conductance regulator; CFTR; ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channelEntrez Gene (Human): 1080Entrez Gene (Mouse) : 12638Entrez Gene (Rat) : 24255SwissProt (Human): P13569SwissProt (Mouse) : P26361SwissProt (Rat) : P34158Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CFTR expression in HEK293T (A), Hela (B), rat spleen (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of CFTR staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.