Description: Rabbit polyclonal antibody to CENPA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CENPA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CENPA Alternative Names: Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-AEntrez Gene (Human): 1058Entrez Gene (Mouse) : 12615SwissProt (Human): P49450SwissProt (Mouse) : O35216Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CENPA expression in HEK293T (A), DLD (B) whole cell lysates.
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  Immunohistochemical analysis of CENPA staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.