Description: Rabbit polyclonal antibody to p16 INK4a (Phospho-S152) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S152 of human p16 INK4a protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDKN2A Alternative Names: CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4AEntrez Gene (Human): 1029SwissProt (Human): P42771Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p16 INK4a (Phospho-S152) expression in HeLa UV-treated (A) whole cell lysates.
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Immunohistochemical analysis of p16 INK4a (Phospho-S152) staining in human cervix cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.