Description: Rabbit polyclonal antibody to CDC25A (Phospho-S75) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S75 of human CDC25A protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDC25A Alternative Names: M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25AEntrez Gene (Human): 993SwissProt (Human): P30304Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CDC25A (Phospho-S75) expression in HeLa UV-treated (A), mouse liver (B) whole cell lysates.
-
Immunohistochemical analysis of CDC25A (Phospho-S75) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation
Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells