Description: Rabbit polyclonal antibody to CD274 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD274. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CD274 Alternative Names: B7H1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274Entrez Gene (Human): 29126Entrez Gene (Mouse) : 60533SwissProt (Human): Q9NZQ7SwissProt (Mouse) : Q9EP73Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD274 expression in mouse lung (A) whole cell lysates.
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  Immunohistochemical analysis of CD274 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.