Description: Rabbit polyclonal antibody to CCR4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CCR4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CCR4 Alternative Names: CMKBR4; C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194Entrez Gene (Human): 1233Entrez Gene (Mouse) : 12773SwissProt (Human): P51679SwissProt (Mouse) : P51680Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CCR4 expression in H446 (A) whole cell lysates.
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Immunohistochemical analysis of CCR4 staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.