Description: Rabbit polyclonal antibody to CaMK2 alpha/delta (Phospho-T286) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T286 of human CaMK2 alpha/delta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: CAMK2A; CAMK2D Alternative Names: CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit deltaEntrez Gene (Human): 815; 817Entrez Gene (Mouse) : 12322; 108058Entrez Gene (Rat) : 25400; 24246SwissProt (Human): Q9UQM7; Q13557SwissProt (Mouse) : P11798; Q6PHZ2SwissProt (Rat) : P11275; P15791Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CaMK2 alpha/delta (Phospho-T286) expression in HEK293T (A), H1792 (B), HepG2 (C), Panc1 (D), AML12 (E) whole cell lysates.
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Immunohistochemical analysis of CaMK2 alpha/delta (Phospho-T286) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of CaMK2 alpha/delta (Phospho-T286) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).