Description: Rabbit polyclonal antibody to CaMK1 alpha (pT177) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T177 of human CaMK1 alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: CAMK1 Alternative Names: Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alphaEntrez Gene (Human): 8536Entrez Gene (Mouse) : 52163Entrez Gene (Rat) : 171503SwissProt (Human): Q14012SwissProt (Mouse) : Q91YS8SwissProt (Rat) : Q63450Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CaMK1 alpha (Phospho-T177) expression in Jurkat Insulin-treated (A) whole cell lysates.
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  Immunohistochemical analysis of CaMK1 alpha (Phospho-T177) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of CaMK1 alpha (Phospho-T177) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).