Description: Rabbit polyclonal antibody to BATF Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BATF. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: BATF Alternative Names: Basic leucine zipper transcriptional factor ATF-like; B-cell-activating transcription factor; B-ATF; SF-HT-activated gene 2 protein; SFA-2Entrez Gene (Human): 10538Entrez Gene (Mouse) : 53314Entrez Gene (Rat) : 299206SwissProt (Human): Q16520SwissProt (Mouse) : O35284SwissProt (Rat) : D4A7E1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BATF expression in HeLa (A), H9C2 (B) whole cell lysates.
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Immunohistochemical analysis of BATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.