Description: Rabbit polyclonal antibody to ATF5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATF5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ATF5 Alternative Names: ATFX; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFxEntrez Gene (Human): 22809Entrez Gene (Mouse) : 107503Entrez Gene (Rat) : 282840SwissProt (Human): Q9Y2D1SwissProt (Mouse) : O70191SwissProt (Rat) : Q6P788Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATF5 expression in HEK293T (A), A549 (B), H1688 (C), mouse heart (D), mouse kidney (E), rat heart (F), rat kidney (G) whole cell lysates.
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  Immunohistochemical analysis of ATF5 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.