Description: Rabbit polyclonal antibody to ANKRD30A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ANKRD30A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ANKRD30A Alternative Names: Ankyrin repeat domain-containing protein 30A; Serologically defined breast cancer antigen NY-BR-1Entrez Gene (Human): 91074SwissProt (Human): Q9BXX3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ANKRD30A expression in SHSY5Y (A), NIH3T3 (B) whole cell lysates.
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Immunohistochemical analysis of ANKRD30A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.