Description: Rabbit polyclonal antibody to ALP Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ALP. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ALPP; ALPPL2 Alternative Names: ALPP; PLAP; Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; ALPPL2; ALPPL; Alkaline phosphatase, placental-like; ALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like; PLAP-likeEntrez Gene (Human): 250; 251Entrez Gene (Mouse) : 11650SwissProt (Human): P05187; P10696SwissProt (Mouse) : P24823Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ALP expression in HEK293T (A), Raw264.7 (B) whole cell lysates.
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Immunohistochemical analysis of ALP staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.