Description: Rabbit polyclonal antibody to Smooth Muscle Actin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Smooth Muscle Actin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ACTA2 Alternative Names: ACTSA; ACTVS; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 proteinEntrez Gene (Human): 59Entrez Gene (Mouse) : 11475Entrez Gene (Rat) : 81633SwissProt (Human): P62736SwissProt (Mouse) : P62737SwissProt (Rat) : P62738Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Smooth Muscle Actin expression in HEK293T (A), Hela (B), A2780 (C), mouse spleen (D) whole cell lysates.
• 
  Immunohistochemical analysis of Smooth Muscle Actin staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.