Description: Rabbit polyclonal antibody to TFCP2L1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TFCP2L1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: TFCP2L1 Alternative Names: CRTR1; LBP9; Transcription factor CP2-like protein 1; CP2-related transcriptional repressor 1; CRTR-1; Transcription factor LBP-9Entrez Gene (Human): 29842Entrez Gene (Mouse) : 81879SwissProt (Human): Q9NZI6SwissProt (Mouse) : Q3UNW5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TFCP2L1 expression in HEK293T (A) whole cell lysates.
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  Immunofluorescent analysis of TFCP2L1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).