Description: Rabbit polyclonal antibody to REG4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human REG4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: REG4 Alternative Names: GISP; RELP; Regenerating islet-derived protein 4; REG-4; Gastrointestinal secretory protein; REG-like protein; Regenerating islet-derived protein IV; Reg IVEntrez Gene (Human): 83998Entrez Gene (Mouse) : 67709Entrez Gene (Rat) : 445583SwissProt (Human): Q9BYZ8SwissProt (Mouse) : Q9D8G5SwissProt (Rat) : Q68AX7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of REG4 expression in mouse pancreas (A) whole cell lysates.
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Immunohistochemical analysis of REG4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.