Description: Rabbit polyclonal antibody to POLR3G Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR3G. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: POLR3G Alternative Names: DNA-directed RNA polymerase III subunit RPC7; RNA polymerase III subunit C7; DNA-directed RNA polymerase III subunit G; RNA polymerase III 32 kDa subunit; RPC32Entrez Gene (Human): 10622Entrez Gene (Mouse) : 67486SwissProt (Human): O15318SwissProt (Mouse) : Q6NXY9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of POLR3G expression in A375 (A), EC9706 (B), C6 (C), BV2 (D), mouse testis (E) whole cell lysates.
-
Immunohistochemical analysis of POLR3G staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.