Description: Rabbit polyclonal antibody to Neuroligin 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Neuroligin 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: NLGN1 Alternative Names: KIAA1070; Neuroligin-1Entrez Gene (Human): 22871Entrez Gene (Mouse) : 192167Entrez Gene (Rat) : 116647SwissProt (Human): Q8N2Q7SwissProt (Mouse) : Q99K10SwissProt (Rat) : Q62765Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Neuroligin 1 expression in HEK293T (A), H446 (B), A549 (C), rat muscle (D) whole cell lysates.
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Immunohistochemical analysis of Neuroligin 1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.