Description: Rabbit polyclonal antibody to LPHN1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LPHN1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: LPHN1 Alternative Names: KIAA0821; LEC2; Latrophilin-1; Calcium-independent alpha-latrotoxin receptor 1; CIRL-1; Lectomedin-2Entrez Gene (Human): 22859Entrez Gene (Mouse) : 330814Entrez Gene (Rat) : 65096SwissProt (Human): O94910SwissProt (Mouse) : Q80TR1SwissProt (Rat) : O88917Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of LPHN1 expression in rat kidney (A), Hela (B) whole cell lysates.
-
Immunofluorescent analysis of LPHN1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.