Description: Rabbit polyclonal antibody to GASP2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GASP2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GPRASP2 Alternative Names: G-protein coupled receptor-associated sorting protein 2; GASP-2Entrez Gene (Human): 100528062; 114928Entrez Gene (Mouse) : 245607SwissProt (Human): Q96D09SwissProt (Mouse) : Q8BUY8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GASP2 expression in A549 (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
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Immunohistochemical analysis of GASP2 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.