Description: Rabbit polyclonal antibody to EYA1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EYA1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EYA1; EYA4 Alternative Names: EYA1; Eyes absent homolog 1; EYA4; Eyes absent homolog 4Entrez Gene (Human): 2138; 2070SwissProt (Human): Q99502; O95677SwissProt (Mouse) : P97767; Q9Z191Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EYA1 expression in PC3 (A), mouse brain (B), mouse testis (C), rat brain (D), rat testis (E) whole cell lysates.
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Immunohistochemical analysis of EYA1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.