Description: Rabbit polyclonal antibody to EPHA1 (Phospho-Y605) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y605 of human EPHA1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EPHA1 Alternative Names: EPH; EPHT; EPHT1; Ephrin type-A receptor 1; hEpha1; EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPHEntrez Gene (Human): 2041Entrez Gene (Mouse) : 13835SwissProt (Human): P21709SwissProt (Mouse) : Q60750Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EPHA1 (Phospho-Y605) expression in A549 (A), PC12 (B) whole cell lysates.
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Immunohistochemical analysis of EPHA1 (Phospho-Y605) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.