Description: Rabbit polyclonal antibody to EIF2S2 (Phospho-S67) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S67 of human EIF2S2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EIF2S2 Alternative Names: EIF2B; Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit beta; eIF-2-betaEntrez Gene (Human): 8894Entrez Gene (Mouse) : 67204SwissProt (Human): P20042SwissProt (Mouse) : Q99L45Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF2S2 (Phospho-S67) expression in DLD (A) whole cell lysates.
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Immunohistochemical analysis of EIF2S2 (Phospho-S67) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.