Description: Rabbit polyclonal antibody to DNMT3A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNMT3A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DNMT3A Alternative Names: DNA (cytosine-5)-methyltransferase 3A; Dnmt3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIAEntrez Gene (Human): 1788Entrez Gene (Mouse) : 13435Entrez Gene (Rat) : 444984SwissProt (Human): Q9Y6K1SwissProt (Mouse) : O88508SwissProt (Rat) : Q1LZ53Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNMT3A expression in MEF (A), H9C2 (B), HCT116 (C), Jurkat (D), PC3 (E), HEK293T (F) whole cell lysates.
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  Immunohistochemical analysis of DNMT3A staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.