Description: Rabbit polyclonal antibody to CD234 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD234. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: DARC Alternative Names: ACKR1; FY; GPD; Duffy antigen/chemokine receptor; Atypical chemokine receptor 1; Fy glycoprotein; GpFy; Glycoprotein D; Plasmodium vivax receptor; CD234Entrez Gene (Human): 2532Entrez Gene (Mouse) : 13349SwissProt (Human): Q16570SwissProt (Mouse) : Q9QUI6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD234 expression in HeLa (A), K562 (B), Raw264.7 (C), PC12 (D) whole cell lysates.
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Immunofluorescent analysis of CD234 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).