Description: Rabbit polyclonal antibody to Calpain 3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Calpain 3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CAPN3 Alternative Names: CANP3; CANPL3; NCL1; Calpain-3; Calcium-activated neutral proteinase 3; CANP 3; Calpain L3; Calpain p94; Muscle-specific calcium-activated neutral protease 3; New calpain 1; nCL-1Entrez Gene (Human): 825Entrez Gene (Mouse) : 12335Entrez Gene (Rat) : 29155SwissProt (Human): P20807SwissProt (Mouse) : Q64691SwissProt (Rat) : P16259Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Calpain 3 expression in mouse muscle (A), rat muscle (B) whole cell lysates.
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Immunohistochemical analysis of Calpain 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.