Description: Rabbit polyclonal antibody to Synaptotagmin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Synaptotagmin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SYT1; SYT2 Alternative Names: SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytIIEntrez Gene (Human): 6857; 127833Entrez Gene (Mouse) : 20979; 20980Entrez Gene (Rat) : 25716; 24805SwissProt (Human): P21579; Q8N9I0SwissProt (Mouse) : P46096; P46097SwissProt (Rat) : P21707; P29101Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Synaptotagmin expression in mouse brain (A), rat muscle (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of Synaptotagmin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.