Description: Rabbit polyclonal antibody to STAT5 (Phospho-Y694/699) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y694/699 of human STAT5 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: STAT5A; STAT5B Alternative Names: STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5BEntrez Gene (Human): 6776; 6777Entrez Gene (Mouse) : 20850; 20851Entrez Gene (Rat) : 24918; 25126SwissProt (Human): P42229; P51692SwissProt (Mouse) : P42230; P42232SwissProt (Rat) : Q62771; P52632Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of STAT5 (Phospho-Y694/699) expression in K562 (A) whole cell lysates.
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  Immunohistochemical analysis of STAT5 (Phospho-Y694/699) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.