Description: Rabbit polyclonal antibody to AMPK alpha 1/2 (Phospho-T183/172) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/172 of human AMPK alpha 1/2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PRKAA1; PRKAA2 Alternative Names: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinaseEntrez Gene (Human): 5562; 5563Entrez Gene (Mouse) : 105787; 108079Entrez Gene (Rat) : 65248; 78975SwissProt (Human): Q13131; P54646SwissProt (Mouse) : Q5EG47; Q8BRK8SwissProt (Rat) : P54645; Q09137Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AMPK alpha 1/2 (Phospho-T183/172) expression in Jurkat Adriamycin-treated (A), K562 Adriamycin-treated (B) whole cell lysates.
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  Immunohistochemical analysis of AMPK alpha 1/2 (Phospho-T183/172) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-AMPK alpha 1/2 (Phospho-T183/172) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.