Description: Rabbit polyclonal antibody to NFIA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFIA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NFIA Alternative Names: NFIA; KIAA1439; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein; NFIB; Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/B; NF-I/B; NFI-B; TGGCA-binding protein; NFIC; NFI; Nuclear factor 1 C-type; NF1-C; Nuclear factor 1/C; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein; NFIX; Nuclear factor 1 X-type; NF1-X; Nuclear factor 1/X; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/X; NF-I/X; NFI-X; TGGCA-binding proteinEntrez Gene (Human): 4774; 4781; 4782; 4784Entrez Gene (Mouse) : 18027; 18028; 18029; 18032Entrez Gene (Rat) : 25492SwissProt (Human): Q12857; O00712; P08651; Q14938SwissProt (Mouse) : Q02780; P97863; P70255; P70257SwissProt (Rat) : P09414Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of NFIA expression in LO2 (A), C6 (B), A2780 (C) whole cell lysates.
• 
  Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.