Description: Rabbit polyclonal antibody to DUSP1/4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DUSP1/4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: DUSP1; DUSP4 Alternative Names: DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4; MKP2; VH2; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2; MAP kinase phosphatase 2; MKP-2Entrez Gene (Human): 1843; 1846Entrez Gene (Mouse) : 19252; 319520Entrez Gene (Rat) : 114856; 60587SwissProt (Human): P28562; Q13115SwissProt (Mouse) : P28563; Q8BFV3SwissProt (Rat) : Q64623; Q62767Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DUSP1/4 expression in HeLa (A), Jurkat (B) whole cell lysates.
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Immunohistochemical analysis of DUSP1/4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.