Description: Rabbit polyclonal antibody to EEF2K (Phospho-S366) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S366 of human EEF2K protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: EEF2K Alternative Names: Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinaseEntrez Gene (Human): 101930123; 29904Entrez Gene (Rat) : 25435SwissProt (Human): O00418SwissProt (Rat) : P70531Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EEF2K (Phospho-S366) expression in DLD (A), rat muscle (B) whole cell lysates.
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Immunohistochemical analysis of EEF2K (Phospho-S366) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.