Description: Rabbit polyclonal antibody to PNPT1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PNPT1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PNPT1 Alternative Names: PNPASE; Polyribonucleotide nucleotidyltransferase 1 mitochondrial; 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like proteinEntrez Gene (Human): 87178Entrez Gene (Mouse) : 71701SwissProt (Human): Q8TCS8SwissProt (Mouse) : Q8K1R3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PNPT1 expression in PC12 (A), MEF (B), A549 (C), LO2 (D), HCT116 (E) whole cell lysates.
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Immunohistochemical analysis of PNPT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.