Description: Rabbit polyclonal antibody to CDK5RAP3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDK5RAP3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDK5RAP3 Alternative Names: IC53; CDK5 regulatory subunit-associated protein 3; CDK5 activator-binding protein C53; Protein HSF-27Entrez Gene (Human): 80279SwissProt (Human): Q96JB5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDK5RAP3 expression in HeLa (A), A431 (B) whole cell lysates.
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Immunohistochemical analysis of CDK5RAP3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.