Description: Rabbit polyclonal antibody to SHARP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SHARP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: BHLHE41 Alternative Names: BHLHB3; DEC2; SHARP1; Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1Entrez Gene (Human): 79365Entrez Gene (Mouse) : 79362SwissProt (Human): Q9C0J9SwissProt (Mouse) : Q99PV5SwissProt (Rat) : O35779Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SHARP1 expression in SHSY5Y (A), RAW264.7 (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.