Description: Rabbit polyclonal antibody to Histone Deacetylase 8 (Phospho-S39) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S39 of human Histone Deacetylase 8 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: HDAC8 Alternative Names: HDACL1; Histone deacetylase 8; HD8Entrez Gene (Human): 55869Entrez Gene (Mouse) : 70315Entrez Gene (Rat) : 100911968; 363481SwissProt (Human): Q9BY41SwissProt (Mouse) : Q8VH37SwissProt (Rat) : B1WC68Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone Deacetylase 8 (Phospho-S39) expression in A549 (A), NIH3T3 (B), rat kidney (C) whole cell lysates.
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  Immunohistochemical analysis of Histone Deacetylase 8 (Phospho-S39) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Histone Deacetylase 8 (Phospho-S39) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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  Direct ELISA antibody dose-response curve using Anti-Histone Deacetylase 8 (Phospho-S39) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.